Journal: Neural Regeneration Research

Article Title: Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice

doi: 10.4103/NRR.NRR-D-23-01742

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: GAPDH antibody , Mouse , Monoclonal , sc-365062 , Santa Cruz Biotechnology, Dallas, TX, USA , AB_10847862 , WB (1:1000).

Techniques:

Journal: bioRxiv

Article Title: Epitranscriptome-wide profiling identifies RNA editing events regulated by ADAR1 that are associated with DNA repair mechanisms in human TK6 cells

doi: 10.1101/2025.07.11.664482

Figure Lengend Snippet: (A) Domain structure of the two ADAR1 isoforms, p150 and p110. (B) Schematic representation of the CRISPR/Cas9-mediated disruption of ADAR1 p150 and p150/p110. The target sequence and corresponding vectors containing either a reverse-oriented puromycin- or neomycin- resistance cassette are illustrated. (C) Western blot analysis of ADAR1 isoforms. Whole-cell extracts from WT, p150 KO clones (#1, #2, #3), and p150/p110 KO clones (#1, #2, #3) were resolved by 10% SDS-PAGE. GAPDH was used as a loading control. (D) Clustering analysis of genes exhibiting A- to-I editing. Based on inosine peak scores (fold-enrichment ratio) in WT and p150 KO cells, genes were categorized as strongly p150 dependent (WT/(p150KO+1)≧4; purple), mildly p150 dependent (11, cyan). Asterisk (*) indicates the genes categorized as p150/p110 KO-specific (WT=0, p150KO=0, p150/p110KO>1).

Article Snippet: To detect ADAR1 and GAPDH, membranes were incubated overnight at 4°C in Hikari A solution (Nacalai Tesque) or 3% skim milk with 1:1000 dilution of anti-ADAR1 monoclonal antibody (D7E2M, Cell Signaling) or 1:1000 dilution of anti-GAPDH monoclonal antibody (sc-32233, Santa Cruz), respectively.

Techniques: CRISPR, Disruption, Sequencing, Western Blot, Clone Assay, SDS Page, Control